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Amaxa basic parasite nucleofector kit
Basic Parasite Nucleofector Kit, supplied by Amaxa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nucleofector+basic+parasite+kit/basic+kit+nucleofector/pm41634752-59-6-10
Average 86 stars, based on 1 article reviews
basic parasite nucleofector kit - by Bioz Stars, 2026-10
86/100 stars

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Transfection:

Article Title: The Dual Role of the 16mer Motif Within the 3' Untranslated Region of the Variant Surface Glycoprotein of Trypanosoma brucei.
Article Snippet: Cell numbers were monitored by counting with a haemocytometer or Z2 Coulter counter (Beckman Coulter). .. For transfections to generate transgenic cell lines, 3 × 107 T. brucei 13–90 cells were electroporated with 10 μg of linearised plasmid DNA using the Amaxa Basic Parasite Nucleofector Kit 1 and Nucleofector II device (programme X001) (Lonza, Switzerland). ..

Article Title: The Dual Role of the 16mer Motif Within the 3′ Untranslated Region of the Variant Surface Glycoprotein of Trypanosoma brucei
Article Snippet: Cell numbers were monitored by counting with a haemocytometer or Z2 Coulter counter (Beckman Coulter). .. For transfections to generate transgenic cell lines, 3 × 10 7 T. brucei 13–90 cells were electroporated with 10 μg of linearised plasmid DNA using the Amaxa Basic Parasite Nucleofector Kit 1 and Nucleofector II device (programme X‐001) (Lonza, Switzerland). ..

Article Title: Roles and Cellular Localization of GBP2 and NAB2 During the Blood Stage of Malaria Parasites
Article Snippet: .. Transfection was performed using an Amaxa Basic Parasite Nucleofector Kit (Amaxa GmbH, Cologne, Germany) according to the manufacturer’s protocol ( ; ). ..

Article Title: Characterization of a nuclear transport factor 2-like domain-containing protein in Plasmodium berghei.
Article Snippet: .. Transfection was performed using an Amaxa Basic Parasite Nucleofector Kit (Amaxa GmbH, Cologne, Germany) according to the manufacturer’s protocol [19]. ..

Article Title: Identification of serine/arginine-rich splicing factor 1-interacting proteins in Plasmodium berghei
Article Snippet: .. Transfection was performed using an Amaxa Basic Parasite Nucleofector Kit (Amaxa GmbH, Cologne, Germany) according to the manufacturer’s protocol as described previously [ , ]. .. Genomic DNA of malaria parasites was extracted from peripheral blood of mice infected with malaria parasites using QIAamp DNA mini kit (Qiagen, Hilden, Germany).

Article Title: Characterization of a nuclear transport factor 2-like domain-containing protein in Plasmodium berghei
Article Snippet: .. Transfection was performed using an Amaxa Basic Parasite Nucleofector Kit (Amaxa GmbH, Cologne, Germany) according to the manufacturer’s protocol [ ]. ..

Article Title: The nucleolar DExD/H protein Hel66 is involved in ribosome biogenesis in Trypanosoma brucei
Article Snippet: .. All transfections for generation of transgenic cell lines were carried out with 3 × 10 7 trypanosome cells, electroporated with 10 μg of linearised plasmid DNA or PCR product using the Amaxa Basic Parasite Nucleofector Kit 1 and Nucleofector II device (Lonza, Switzerland, program X-001). .. Whole cell protein lysate from 1 × 10 6 trypanosome cells was separated on 12.5% sodium dodecyl sulphate (SDS)-polyacrylamide gels and transferred onto nitrocellulose membranes (GE Healthcare Life Sciences).

Article Title: Identification of serine/arginine-rich splicing factor 1-interacting proteins in Plasmodium berghei.
Article Snippet: .. Transfection was performed using an Amaxa Basic Parasite Nucleofector Kit (Amaxa GmbH, Cologne, Germany) according to the manufacturer’s protocol as described previously [13, 14]. .. Genomic DNA of malaria parasites was extracted from peripheral blood of mice infected with malaria parasites using QIAamp DNA mini kit (Qiagen, Hilden, Germany).

Transgenic Assay:

Article Title: The Dual Role of the 16mer Motif Within the 3' Untranslated Region of the Variant Surface Glycoprotein of Trypanosoma brucei.
Article Snippet: Cell numbers were monitored by counting with a haemocytometer or Z2 Coulter counter (Beckman Coulter). .. For transfections to generate transgenic cell lines, 3 × 107 T. brucei 13–90 cells were electroporated with 10 μg of linearised plasmid DNA using the Amaxa Basic Parasite Nucleofector Kit 1 and Nucleofector II device (programme X001) (Lonza, Switzerland). ..

Article Title: The Dual Role of the 16mer Motif Within the 3′ Untranslated Region of the Variant Surface Glycoprotein of Trypanosoma brucei
Article Snippet: Cell numbers were monitored by counting with a haemocytometer or Z2 Coulter counter (Beckman Coulter). .. For transfections to generate transgenic cell lines, 3 × 10 7 T. brucei 13–90 cells were electroporated with 10 μg of linearised plasmid DNA using the Amaxa Basic Parasite Nucleofector Kit 1 and Nucleofector II device (programme X‐001) (Lonza, Switzerland). ..

Article Title: The nucleolar DExD/H protein Hel66 is involved in ribosome biogenesis in Trypanosoma brucei
Article Snippet: .. All transfections for generation of transgenic cell lines were carried out with 3 × 10 7 trypanosome cells, electroporated with 10 μg of linearised plasmid DNA or PCR product using the Amaxa Basic Parasite Nucleofector Kit 1 and Nucleofector II device (Lonza, Switzerland, program X-001). .. Whole cell protein lysate from 1 × 10 6 trypanosome cells was separated on 12.5% sodium dodecyl sulphate (SDS)-polyacrylamide gels and transferred onto nitrocellulose membranes (GE Healthcare Life Sciences).

Plasmid Preparation:

Article Title: The Dual Role of the 16mer Motif Within the 3' Untranslated Region of the Variant Surface Glycoprotein of Trypanosoma brucei.
Article Snippet: Cell numbers were monitored by counting with a haemocytometer or Z2 Coulter counter (Beckman Coulter). .. For transfections to generate transgenic cell lines, 3 × 107 T. brucei 13–90 cells were electroporated with 10 μg of linearised plasmid DNA using the Amaxa Basic Parasite Nucleofector Kit 1 and Nucleofector II device (programme X001) (Lonza, Switzerland). ..

Article Title: The Dual Role of the 16mer Motif Within the 3′ Untranslated Region of the Variant Surface Glycoprotein of Trypanosoma brucei
Article Snippet: Cell numbers were monitored by counting with a haemocytometer or Z2 Coulter counter (Beckman Coulter). .. For transfections to generate transgenic cell lines, 3 × 10 7 T. brucei 13–90 cells were electroporated with 10 μg of linearised plasmid DNA using the Amaxa Basic Parasite Nucleofector Kit 1 and Nucleofector II device (programme X‐001) (Lonza, Switzerland). ..

Article Title: The nucleolar DExD/H protein Hel66 is involved in ribosome biogenesis in Trypanosoma brucei
Article Snippet: .. All transfections for generation of transgenic cell lines were carried out with 3 × 10 7 trypanosome cells, electroporated with 10 μg of linearised plasmid DNA or PCR product using the Amaxa Basic Parasite Nucleofector Kit 1 and Nucleofector II device (Lonza, Switzerland, program X-001). .. Whole cell protein lysate from 1 × 10 6 trypanosome cells was separated on 12.5% sodium dodecyl sulphate (SDS)-polyacrylamide gels and transferred onto nitrocellulose membranes (GE Healthcare Life Sciences).

Polymerase Chain Reaction:

Article Title: The nucleolar DExD/H protein Hel66 is involved in ribosome biogenesis in Trypanosoma brucei
Article Snippet: .. All transfections for generation of transgenic cell lines were carried out with 3 × 10 7 trypanosome cells, electroporated with 10 μg of linearised plasmid DNA or PCR product using the Amaxa Basic Parasite Nucleofector Kit 1 and Nucleofector II device (Lonza, Switzerland, program X-001). .. Whole cell protein lysate from 1 × 10 6 trypanosome cells was separated on 12.5% sodium dodecyl sulphate (SDS)-polyacrylamide gels and transferred onto nitrocellulose membranes (GE Healthcare Life Sciences).



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